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Dna polymerase iii holoenzyme is the primary enzyme complex involved in prokaryotic dna replication Dna polymerase iii holoenzyme contains two dna polymerases embedded in a particle with 9 other subunits It was discovered by thomas kornberg (son of arthur kornberg) and malcolm gefter in 1970.
Dna polymerase can only initiate the addition of nucleotide if there is a preexisting 3’ end This multi subunit dna polymerase is the escherichia coli chromosomal replicase, and it has several special features that distinguish it as a replicating machine. Therefore, dna polymerase needs a preexisting short stretch of a nucleotide called primer, to provide that 3’ end.
By itself, the polymerase catalytic subunit of the dna polymerase iii holoenzyme (pol iii he), a, exhibits no special properties that hint of the pol iii he’s high catalytic efficiency, accuracy, and enormous processivity.
Dna polymerase iii (pol iii) is encoded by the polc gene and is the primary replication enzyme in e Dna pol iii consists of three core subunits (α, ε, and θ) and several accessory subunits. The dimeric structure of the dna polymerase iii holoenzyme couples leading and lagging strand dna syntheses during replication Leading strand synthesis (bottom strand) is continuous and processive, but lagging strand synthesis must reinitiate many times during replication of the chromosome.
The dna polymerase iii holoenzyme, a central player in prokaryotic dna replication, is a multifaceted complex composed of several distinct subunits, each with its specific function. Dna polymerases have a complex structure, allowing them to carry out their dna replication and repair functions The structure of dna polymerase can vary slightly among different organisms and types of polymerases, but they generally share standard features. 172 kelman & o'donnell abstract dna polymerase iii holoenzyme contains two dna polymerases embedded in a particle with 9 other subunits
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